Sorghum Hybrids and Their Parental Genetic Differences in Diversity

Objective. In order to further prove the diversity of genes in the leaves of hybrid and parental leaves of sorghum. Method. Four copies of the sorghum sterile lines and five types of Sudan grass for parents, according to the NC Ⅱ design，were formulated to 20 hybrid combinations. Measuring the cross combinations and field shape index and physiological and biochemical index of the parents.Using cDNA-AFLP differential display analysis of gene diversity difference. Result. The results of 29 sorghum hybrids and their parental cDNA-AFLP markers for the hybrids and their parental leaves were displayed:12 pairs of primer combinations amplified 336 sites, including specific sites 276, accounting for 82.14% of the total, an average of primer combinations amplified specific sites 23.The number of alleles (No) was 1.8701, the effective number of alleles (Ne) was 1.7365, the Nei’s gene diversity index (H) was 0.3898, and the Shannon’s (I) was 0.5569, which indicated that the gene expression of the hybrid and its parents were complex.The results of cluster analysis show that the change of Genetic similarity coefficient between 29 sorghum sudanense materials is between 0.508-0.876, and the Genetic similarity coefficient 0.54 is the standard, and the 29 materials can be divided into 4 categories.The parental materials were mainly in Ⅰand the Ⅳ class, and the hybrids were mainly in the Ⅱ and Ⅲ. Conclusion. The reactive system of cDNA-AFLP differential display technique was constructed, and the genetic diversity of the leaves in the seedling stage was analyzed.