P1049
Large-Scale Analysis of Pedigree and Sperm-Typing Data Reveals PRDM9 Allele-Specific Recombination Maps in Cattle

Botong Shen , Department of Animal and Avian Sciences, University of Maryland, College Park, MD
Yang Zhou , Animal Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD
Jicai Jiang , Department of Animal and Avian Sciences, University of Maryland, College Park, MD
Abinash Padhi , Department of Animal and Avian Sciences, University of Maryland, College Park, MD
Ki-Eun Park , Department of Animal and Avian Sciences, University of Maryland, College Park, MD
Bhanu Telugu , Department of Animal and Avian Sciences, University of Maryland, College Park, MD
Hong Chen , College of Animal Science and Technology, Northwest A&F University, Shaanxi, China
George E. Liu , Animal Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD
John B. Cole , Animal Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD
Li Ma , Department of Animal and Avian Sciences, University of Maryland, College Park, MD
Meiotic recombination is a major driving force in promoting genetic and phenotypic variations in sexually reproducing organisms. Although PRDM9 is known to modulate the binding-specificity and location of recombination hotspots in humans and mice, its role, especially in domesticated animals like cattle, is largely uncharacterized. Using large-scale pedigree data and sperm-typing techniques, here we report PRDM9 allele-specific recombination maps and hotspots across the cattle genome, dominant effects of PRDM9 alleles on recombination patterns, and strong associations between these hotspots and unique mutations at functional sites in the PRDM9 zinc fingers. Comparing recombination maps built from sperms and pedigree data of a single bull, we identified two regions with slightly different recombination rate, although the genome-wide recombination rate is the same between sperm and pedigree. Our results provided an example for the construction of recombination maps for domesticated animals and enhanced our understanding of the impacts of PRDM9 on meiotic recombination.
Back to: Genome Mapping, Tagging & Characterization: Cattle
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