P0675BEAN_ADAPT: Evolution in a Changing Environment: The Genetic Architecture of Adaptation Outside Centers of Domestication of Phaseolus vulgaris and P. coccineus
The main aim of this project is to dissect out the genetic basis and phenotypic consequences of the adaptation to new environments of the common bean (Phaseolus vulgaris L.) and its sister species, the runner bean (Phaseolus coccineusL.), through the study of their introduction, from their respective centers of domestication in the Americas, and expansion through Europe, as a recent and historically well-defined event of rapid adaptation.
We are characterizing a large collection (11,500 accessions of both species) by genotyping-by-sequencing (GBS), and a deeper phenotypic and molecular characterization of subsets of genotypes is in progress, with field and growth chamber experiments and genomic–transcriptomic–metabolomic analyses. We are using a multidisciplinary approach: genomics (WGS and RNAseq), population/ quantitative genetics, biochemistry, plant physiology on the subset of samples. Differential expression analysis, analysis of the co-expression patterns, and GWAS will be used to identify genes and metabolites putatively associated with adaptation, while genotypic information obtained from RNA-seq data will be used, with GBS and WGS data, to test for signatures of selection.
Among the main outcomes of the project are the development in P. vulgaris of haplotypes of all 10,000 accessions (HapBean), along with associated information and seed stocks, which will represent a unique tool for plant scientists and breeders. For P. coccineus, we will also have a well-defined set of information that will constitute the foundation for the development and application of its genomic resources.
BEAN_ADAPT aspires to work with ‘bean research community’ members, gene banks, plant breeders, stakeholders, plant and crop scientists, and database and computational experts to form a wide collaborative network. Over 20 representatives of Universities, Institutes and Companies have shown interest in collaboration within BEAN_ADAPT and
became Associated Partners.
BEAN_ADAPT proposes to make available technologies and germplasm directly to seed companies and farmers, to implement efficient plant breeding programs and contribute to a multilateral system to access and share in the benefits deriving from research and the use of genetic resources.