Label-free Pollen Viability Determination by Impedance Flow Cytometry

Analysis of pollen viability plays an important role in various aspects of plant breeding and production processes. Here, we show that the label-free analysis of pollen based on their dielectric properties by impedance flow cytometry (IFC) is a very efficient method for the determination of pollen viability and microspore maturation in a high-throughput manner. In addition, we demonstrate the unique potential of IFC in pollen analysis with respect to germinability and ploidy.