De novo Assembly of the Tommy Atkins Mango Genome

Genomic denovo assembly technologies have made a significant progress over the last few years. Having said that, accurate and robust resolution of complex genomes still remains an unmet challenge both algorithmically & economically. Plant genomes are known to be highly diverse in their size, zygosity, ploidy and repetitive elements frequency. These differences exist both intra- & inter- species, a fact which enhance the need for a high throughput, cost effective solution.

Here we present a fully phased assembly project of the heterozygous, polyploid, Tommy Atkins mango. This is a result of the newly developed NRGene’s DeNovoMagic-3 platform using only illumina short reads technology. Using 2 segregating populations maps, we were able to reconstruct 50 linkage groups representing 85% of the total assembly size of approx. 760Mb.