Further Evidence That Mutations in <i>UGT74S1</i>  Alter SDG Lignan Glucoside Profiles in EMS Flax Mutant Lines

Cloutier, Sylvie

P0233
Further Evidence That Mutations in UGT74S1 Alter SDG Lignan Glucoside Profiles in EMS Flax Mutant Lines

Kaushik Ghose , Agriculture and Agri-Food Canada, Charlottetown, PE, Canada

Ashok Somalraju , Agriculture and Agri-Food Canada, Charlottetown, PE, Canada

Jason McCallum , Agriculture and Agri-Food Canada, Charlottetown, PE, Canada

Sylvie Cloutier , Agriculture and Agri-Food Canada, Ottawa, ON, Canada

Bourlaye Fofana , Agriculture and Agri-Food Canada, Charlottetown, PE, Canada

Flax glycosyltransferase UGT74S1 was recently found to sequentially catalyze secoisolariciresinol (SECO) monoglucoside (SMG) and diglucoside (SDG) formation in vitro using the aglycone precursor SECO. However, whether this gene is unique in achieving this glucosylation process in planta is not clear. We developed and characterized a flax EMS mutant population using reverse and forward genetics to assess loss-of-function in UGT74S1. EMS lines carrying missense or nonsense mutations and showing altered lignan profiles were identified. Ampliseq sequencing using a panel of 10 genes encountered in the lignan biosynthetic pathways was performed in EMS lines displaying altered lignan profiles. The data will be reported and discussed in connection with the putative roles for UGT74S3 and UGT74S4, two closely related genes to UGT74S1.

Plant and Animal Genome XXV Conference

January 13 - 18, 2017

P0233
Further Evidence That Mutations in UGT74S1 Alter SDG Lignan Glucoside Profiles in EMS Flax Mutant Lines

Meeting Information

Plant and Animal Genome XXV Conference January 13 - 18, 2017

P0233Further Evidence That Mutations in UGT74S1 Alter SDG Lignan Glucoside Profiles in EMS Flax Mutant Lines

Meeting Information

Plant and Animal Genome XXV Conference

January 13 - 18, 2017

P0233
Further Evidence That Mutations in UGT74S1 Alter SDG Lignan Glucoside Profiles in EMS Flax Mutant Lines