Control of Meiotic Recombination by the Phosphorylation of Chromosome Axis ASY1 Protein

ASY1 is a meiotic specific chromosome axis protein in plants. Mutations on this protein decreases immensenly the number of crossovers events but DSBs are repaired. We have carried out an analysis of putative phosphorylation sites in ASY1 in Arabidopsis thaliana. A construct carrying ASY1 genomic sequence with its promotor and terminator, resulted to be complementing successfully the mutant asy1-4. We have also produced different similar contstructs with point mutations in key residues which more likely phosphorylation would take place and we have started analysing the roles of this phosphosites in ASY1 and their effect in meiotic recombination.