P0046Use of Paramagnetic Particles for the Automated Purification of DNA from Cultured Cells and Mammalian Tissue
Cell populations, traditionally, have been cultured on two dimensional (2D) substrates such as tissue culture polystyrene (TCPS) or the surface of tissue analogs. Mammalian cells growing in a monolayer, however, only establish cell-cell contacts at the periphery where conditions are remarkably distinct from real in vivo situations. Furthermore, it is known that many aspects of the cell microenvironment play a major role in cellular morphology, physiology, metabolism and the response to external stimuli. Three-dimensional (3D) cell cultivation has therefore become an essential and popular platform for researchers looking to more accurately mimic the native cell environment.
Mouse and rat are the most commonly used mammalian model systems to study tissue physiology and pathophysiology. Whether using mice or rats, it is often necessary to confirm the specific genotypes in order to eliminate genetic contamination and to control the quality of the population. This is often does using mouse ear punches or mouse tail clippings.
Here we present an automated approach using novel paramagnetic particles to purify and amplify nucleic acids from DNA 3D cell cultures grown in both low and high cell density growth media and mouse tail or ear punches tissues. These particles are easily automated for low to medium throughput isolation using a small bench-top purification system. Purified DNA was evaluated for amplification in qPCR and for presence of inhibitors.