P1188Molecular Characterisation of Different Genetic Resources of Ferns Grown in Turkey
Ferns, which were known to be exist since ancient periods, were first observed on earth approximately 300 million years ago at the Devonian Era. Considering that no other species were living on earth at that Era, existence of ferns and the diversification of their form becomes a phenomenal subject. Ferns also described as the source of the worlds’ coal beds. With all these facts, to determine the endemic species and the taxa locations become essential both for the agricultural and many other disciplines.
In this study, different genetic resources of ferns grown in certain regions of Turkey. Genetic diversity was investigated using sequence related amplified polymorphism (SRAP) method. These species are: Cheilanthes acrostica, C. persica, Adiantum capillus-veneris, Pteris vittata, Dryopteris filix-mas L., Asplenium onopteris L., A. trichomanes, A. scolopendrium L. subsp. scolopendrium, A. septentrionale, Asplenium haussknechtii, Polypodium vulgare, A. ceterach and A. bourgaei. DNA was successfully amplified by 20 SRAP combinations. Among 364 bands generated by the SRAP primers, 364 were polymorphic. The percentage of polymorphism was 100% based on SRAP data. All data were scored as discrete characters and unweighted pair group method with arithmetic mean (UPGMA) dendrogram and principle coordinate analysis (PCoA) scatter plot were constructed. Based on the results, fern genotypes showed high genetic diversity and we showed that SRAP markers are powerful tool to discriminate Turkish fern genotypes. The high genetic diversity existing in the Turkish germplasm suggests that it would be beneficial to utilize this pool in fern breeding programs and germplasm management activities.