Plant and Animal Genome XXV Conference

January 13 - 18, 2017

4336

Lexogen - QuantSeq 3' mRNA-Seq technology as an efficient tool for gene expression profiling

With the rapid development of NGS technologies, RNA-Seq has become the new standard for transcriptome analysis. Although the price per base has been substantially reduced, sample preparation, sequencing, and data processing still remain major cost factors in high-throughput screenings. The QuantSeq technology addresses these issues by providing an easy protocol to generate highly strand-specific NGS libraries close to the 3' end of polyadenylated RNAs within 4.5 hours, requiring only 0.1 - 500 ng of total RNA input and less bioinformatics efforts than standard RNA-Seq. It is the method of choice for fast, affordable and accurate gene expression detection, quantification and 3’ UTR studies. The protocol can be readily modified for targeted sequencing and include molecular barcoding. In our workshop users of QuantSeq will present their studies where this technology has empowered their discoveries.