Long-duration spaceflight is associated with musculoskeletal, postural, and respiratory adaptations due to altered gravitational loading, yet downstream effects on phonation remain understudied. This pilot acoustic study involves joint analysis between speech-language pathology and orthopedics to examine whether a partial-gravity analog condition is associated with measurable changes in vocal function. Voice samples collected before and during partial-gravity simulation were analyzed using Praat to quantify acoustic parameters including fundamental frequency (f0), jitter, shimmer, harmonics-to-noise ratio, cepstral peak prominence (CPP), spectral tilt, and formant frequencies (F1–F3). By examining interactions among respiratory mechanics, musculoskeletal loading, and voice production under altered loading conditions, we aim to identify preliminary acoustic markers of spaceflight-relevant change in the vocal mechanism. Findings may inform future frameworks for respiratory and voice exercise protocols, vocal health support, and phonatory function countermeasures during long-duration spaceflight missions.