Effective mixing plays a critical role in recovery and purity of biological intermediates from plant extracts by solvent extraction. Phytochlorin e6, a plant-derived component of chlorophyll that is currently in pilot-scale production, is an intermediate for photodynamic therapy of cancer cells. Several purification steps including acetone precipitation are highly dependent on mixing. We evaluate use of in-line mixing to increase micro-mixing and decrease the characteristic mixing length of the system. This method of high velocity mixing has been implemented to improve product purity, recovery, characteristics, and reduce acetone use. We quantitate effects of optimizing tee-mix parameters including relative velocities, pipe diameter, and phytochlorin concentration on purity and recovery of phytochlorin e6.