This preliminary program is subject to change.

Protein Expression and Post-Translational Modifications


Description:
This session will highlight advances in protein expression in a wide variety of platforms, including bacteria, yeast, insect, mammalian, and plant cells as well as cell-free expression systems. Contributions focusing on protein expression and modification in non-model cellular platforms are especially welcome, as are papers pertaining to spatially organized or scaffolded protein complexes. The session will focus on the engineering of expression hosts for improved protein expression as well as on the design of novel methods for protein display, detection of intracellular trafficking, and cell-cell communication. In addition to the focus on protein expression, the session will also include papers on the development of in vivo and in vitro techniques for improved post-translational modification of proteins, including folding, glycosylation, editing and secretion.

Sponsor:
Bioengineering


Chair:
Michelle A. O'Malley
Assistant Professor
University of California, Santa Barbara
Department of Chemical Engineering
Santa Barbara, CA 93106-5080
Email: momalley@engineering.ucsb.edu

Co-Chair:
Mark A. Blenner
Assistant Professor
Clemson University
Department of Chemical & Biomolecular Engineering
Earle Hall 207A
Clemson, SC 29634-0909
Phone Number: 617-713-8222
Email: blenner@clemson.edu


Papers:
48a
Development of a Bacterial System for the Expression of Full-Length Monoclonal Antibodies
Michael-Paul Robinson, Cornell University; Matthew P. DeLisa, Cornell University
48b
Protein Endocytosis and Degradation: Impact On Secreted Protein Titers in Yeast
Keith E.J. Tyo, Northwestern University; Zihe Liu, Chalmers University of Technology; Dina Petranovic, Chalmers University of Technology; Jens Nielsen, Chalmers University of Technology
48c
Engineering G-Protein Coupled Receptor Signaling in Yeast
Patrick M. McNeely, University of Delaware; Anne S. Robinson, University of Delaware
48d
Soluble Human Lysozyme Production in Escherichia Coli Via an Engineered Anti-Toxin Switch
Jonathan Guerrette, Dartmouth College; John W. Lamppa, Dartmouth College; Samuel Tanyos, Dartmouth College; Karl E. Griswold, Dartmouth College
48f
Engineering Synthetic Organelles: Encapsulating Heterologous Proteins Into Bacterial Microcompartments
Edward Y. Kim, University of California; Danielle Tullman-Ercek, University of California, Berkeley
48g
Expression and Characterization of Three Trichoderma Reesei Cellulose Hydrolases in Kluyveromyces Lactis
Michael J. Brodeur-Campbell, Michigan Technological University; David R. Shonnard, Michigan Technological University
48h
Functional Self-Assembly of Artificial Cellulosomes for Efficient Cellulose Hydrolysis
Qing Sun, University of Delaware; Shen-Long Tsai, University of Delaware; Bhawna Madan, University of Delaware; Wilfred Chen, University of Delaware