SMRT® Sequencing Solutions for Large Genomes

Single Molecule, Real-Time (SMRT) Sequencing holds promise for addressing new frontiers in large genome complexities, such as long, highly repetitive, low-complexity regions and duplication events that are difficult to resolve with short-read technologies.  We present solutions available for both reference genome improvement (>100 MB) to best leverage long reads that have exceeded 20 Kb in length.  Benefits for these applications are further realized with consistent use of size-selection of input sample using the BluePippin™ device from Sage Science.  We will share highlights from our genome improvement projects using the latest P5-C3 chemistry on model organisms, which produced assemblies having contig N50 exceeding 6 Mb and longest contig exceeding 12.5 Mb, with an average base quality of QV50.