SMRT® Sequencing Solutions for Plant and Animal Genome Research

PacBio^® Single Molecule, Real-Time (SMRT) Sequencing holds promise for addressing new frontiers in large genome complexities, such as long, highly repetitive, low-complexity regions and duplication events that are difficult to resolve with short-read technologies.  We present solutions for plant genome research that best leverage long reads exceeding 20 Kb in length.  Benefits for these applications are further realized with consistent use of size selection for input sample using the BluePippin™ device from Sage Science.  Highlights from our genome improvement projects using the latest P5-C3 chemistry on model organisms demonstrate contig N50 exceeding 6 Mb and longest contigs exceeding 12.5 Mb with an average base quality of QV50.  Targeted solutions can also be employed to conduct gene surveys and specific characterizations to understand biodiversity of traits.  Our new Iso-Seq™ Application offers direct sequencing of full-length transcripts to understand isoform diversity and improve annotations of genomes.