Genomic and Functional Analysis of Beneficial Soil Microbe for Biofertilizer Development

A bio-fertilizer is a substance which contains living microbes which can colonize inside or outside of a plant and help the plant to grow faster or healthier. Next generation sequencing (NGS) technology made us easy to sequence the genome of the microbes which can be used as an ingredient of bio-fertilizer. Recently, so many microorganisms were sequenced and more numbers of microbes are being sequenced. Genome sequencing can be used for the quality control and improvement of the microorganisms. Bacillus methylotrophicus, designated strain CBMB205, was isolated from the rhizosphere soil of field-grown rice. Cells were Gram-positive and rod-shaped. The genomic DNA was isolated and sequenced using PacBio platform with 259 coverage. Total 77,620 reads were produced, and these were assembled by PacBio SMRT Analysis 2.3.0. The genome of B. methylotrophicus CBMB205 had 3,929,745 base pairs circular chromosome with 46.50% GC contents and consists of 2,846 genes. It contained 27 rRNA and 86 tRNA sequence, 10% and 8% of the total genes involved amino acid transport and metabolism and transcription, respectively. These results were similar to the previously reported genus Bacillusgenome sequences. The genes related to the bio-fertilizer function were analyzed intensively.

This work was funded by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.

Keywords: Bacillus methylotrophicus, genome, rhizosphere, CBMB205, bio-fertilizer

*Corresponding author: leeyi22@hanmail.net