SNP (Single Nucleotide Polymorphism) and SSR (Simple Sequences Repeat) Variation in Common Bean

Common bean (Phaseolus vulgaris L.) is one of the most  important legumes in the world. It is a widely distributed crop of considerable importance in many countries around the world, representing a major protein source in the population’s diet.  In this study, SNP and SSR marker techniques for their ability to detect genetic diversity within a set of 67 common bean genotypes. For this purpose thirty microsatellit loci and forty-eight SNP primers were used to screen for polymorphism among the genotypes. A total of one hundred and twelve bands were scored in seventy eight primers. The number of total bands per primer pair ranged from 1 to 7, with an average of 1.5. The average PIC value for the SNP and SSR products was 0.58. The minimum variation was found between samples 57 England and 60 Holland, at a value of 100%, and the maximum variation was found between sample 33 Turkey and sample 52 and 53 India, at 70%. Unweighted paired group method with arithmetic averages cluster analysis (UPGMA) was used to discriminate the 67 common bean isolates into 5 subgroups. Finally, we discuss that mix of both SNP and SSR analysis confirmed that they are an efficient and effective markers technology for determining genetic relationships among common bean genotypes.