Genetic Diversity and Relationship Among Wild Annual Lens Species Using iPBS-Retrotransposons Markers

This study  was focused to understand  the genetic diversity and relationship among six Lens species. Seventy three iPBS-retransposon based markers and 30 ISSR markers were screened in a preliminary experiment among 8 accessions belonging to different Lens species. Ten informative primers which produced clear, reproducible, and unambiguous fragments were selected for each molecular marker system. These markers were used to search genetic diversity and relationship among fifty accessions belonging to six wild and one cultivated species (Lens culinaris subsp. culinaris). Total 151 scorable bands were detected using 10 iPBS-retransposon primers and out of which 150 bands were polymorphic (99.3%), with an average of 15.1 polymorphic fragments per primer, whereas in case of ISSR markers, total 138 bands were observed with 100% polymorphism with an average of 13.8 bands per primer. According to data obtained from iPBS-retransposon marker, L. culinaris subsp. orientalis and L. ervoides were the most diverse species, whereas L. lamottei exhibited lower polymorphism. The average polymorphism information contents (PIC) values were 0.90 and 0.97 for iPBS-retrotransposon and ISSR marker systems, respectively. The clustering of the accessions and species within groups was almost similar, when iPBS and ISSR NeighborNet (NNet) planar graph were compared. The tree based on ISSR and iPBS-retransposon markers turned out to be informative regarding relationships among species. The present analysis clearly revealed the close relationships between Lens culinaris subsp. culinaris and Lens culinaris subsp. orientalis.