P0101
Libraries Preparation Automation on a TECAN EVO200

Date: Monday, January 14, 2013
Room: Grand Exhibit Hall
Olivier Bouchez , INRA GeT Genomics Facility, Castanet-Tolosan, France
Cécile Donnadieu , Plateforme Genomique, Castanet tolosan, France
Gerald Salin , INRA, UMR444 LGC - GeT-PlaGe Genomics Facility, castanet tolosan, France
Diane Esquerre , Plateforme Genomique, Castanet tolosan, France
Cemile Ant , Tecan, lyon, FL, France
David Rodarie , Tecan, Lyon, France
Denis Milan , INRA, castanet tolosan, France
Growing output of Next Generation Sequencers allows to sequence at the same time, in a single run, more and more samples. If we want benefit from this very high throughput, we have to achieve a high multiplexing level, the bottleneck being the manual preparation of the librairies. To get rid of this time-consuming task, we developped, with TECAN, an automated process to produce more librairies on the same time on our EVO200. Currently, one script is validated for TruSeq RNA sample prep kit v2 and others are under development.