Genotyping-By-Sequencing (GBS) In The Real Oat World: Making The Data Talk

Genotyping-by-sequencing (GBS) combines genome complexity reduction, sample multiplexing, and high-throughput sequencing to provide simultaneous highly-parallelized marker discovery and genotyping. GBS shows promise for use in oat, which has a complex genome and relatively sparse existing sequence data. We have used GBS to genotype 746 diverse oat varieties and 622 progenies from 8 bi-parental populations. The number of segregating SNPs inside sub-populations reflects the intra-population diversity, both for bi-parental populations and diversity panels used in different breeding programs. With these data, we are attempting to improve the oat consensus map and to generate predictions regarding breeding value and QTL presence in North American oat germplasm. This work will complement and enhance SNP marker genotypes collected in the same germplasm. We will discuss the relevance of GBS for genotyping and genomic-assisted breeding.