Using NGS Data to Facilitate Plant Breeding

We are developing a platform withing the CLC suite to optimise bulk segregant analysis on the population of plants derived from all possible pairwise crosses of a small number of elite parental lines. Parental DNA, as well as pooled progeny DNA from the individuals having extreme values of a trait of interest, has been sequenced. Implemented outcomes would be facilitation of genome-wide selection, assignment of 'breeding values' to elite parents, and identification of individual markers/genes affecting the trait.