Validation of the VERSA 10 Workstation for Real-Time PCR Setup using cDNA from two Genes of Trout Fish

A major challenge in automated PCR technology is efficiently scaling up to high-throughput PCR using microlitre volumes of cDNA samples while also ensuring low cross-contamination and high accuracy and reproducibility. Aurora Biomed Inc. has recently launched its VERSA 10 PCR Setup Workstation as an answer for accurate and reproducible high-throughput PCR. In this work, real-time PCR setups for 2 trout fish genes (SF10 and SF1a) were configured using Aurora Biomed’s VERSA 10 Workstation to dispense 1 or 2 µL-volumes of cDNA template into an alternate column to check for cross-contamination. The statistical analysis from the replicates and inter-channel capabilities indicates that each of the channels reproducibly dispenses accurate volumes along the deck while conditions generate amplicons of the same size, making the VERSA 10 PCR Setup Workstation an excellent alternative for high-throughput and real-time PCR.