Towards Fine Mapping of Drought Tolerance Related QTL Region in Chickpea Using Genotyping by Sequencing (GBS) Approach

Terminal drought is one of the major limitations to chickpea production, which results up to 50% of yield losses. Based on extensive genotyping and phenotyping data on ICC 4958 × ICC 1882 recombinant inbred line (RIL) population, a genomic region (~35cM) harbouring several QTLs for drought tolerance related traits was identified on linkage group 04 (LG 04). This region contained only four markers (TAA170, ICCM0249, STMS11 and GA24).Recently six markers have been mapped in this region using targeted marker development and medicago – chickpea synteny approach. For enhancing the marker density in this region genotyping by sequencing (GBS) approach is being used. In this context, GBS approach (with Apek I enzyme)with the coverage of 5x and 0.2x has been employed on parental lines and 232 RILs respectively. By using stringent criteria genotyping data have been assembled for 828 SNPs on the population. Efforts are underway to construct the genetic map and QTL analysis for the identification of more markers in the QTL region. Updated results will be presented and discussed in the conference.