Shotgun Label Free Quantitative Proteomics of Developing Peanut (Arachis hypogaea L.) Seed

Legume seeds and peanuts, in particular, are an inexpensive source of plant proteins and edible oil. Owing to their importance in global food security, it is necessary to understand the genetic, biochemical, and physiological mechanisms controlling seed quality and nutritive attributes. A comprehensive understanding of seed development and the incorporation of the main storage reserves in seeds, such as proteins, fatty acids, starch, and secondary metabolites will enhance our ability to improve seed quality and yield through molecular breeding programs. In the present study we employed a gel-LC-MS/MS followed by label free quantitation to study the primary transcriptional networks and functional proteins involved in the developing peanut seed at four distinct developmental stages. The presentation will primarily focus on protein profiling to understand key metabolic pathways predominant in each stage of development. Additionally, the effects of water-deficit stress on seed protein, oil quality, and composition at these seed development phases will be discussed.