Partial Genome Annotation of the California Spiny Lobster, Panulirus interruptus, from Baja California, México

The California spiny lobster, Panulirus interruptus, is one of the most important species caught in Baja California. However, there are few studies on its genetics and there are about eight microsatellites for population genetic analysis. In order to make a preliminary genome analysis and to increase the number of genetic markers a high-throughput sequencing (Illumina, MiSeq) was carried out and several bioinformatics programs (CLC-Genomics Workbench, Blast2GO and QDD2) were used for data processing. A total of 5.4 million reads were assembled and produced 204 thousand contigs with an average length of 369 bases, where 57.18% of the reads formed contigs and 42.82% remained as singletons. A preliminary analysis showed a low contig annotation (14%), where the five top-hit species were Nematostella vectensis, Strongylocentrotus purpuratus, Hydra magnipapillata, Aplysia californica, and Capitella teleta. Annotation of biological processes gave a 35% on biosynthetic process and DNA metabolic process, while transferase activity had the 36% in molecular function and protein complex had a 27% in cellular component. Contigs contained 16,773 potential SNPs and 25,915 sequences contained at least one microsatellite. In this paper, we will discuss the annotation results and the potential of SNP and microsatellites for population genetic analysis of this important species in Mexico.