Identification of Gamma-Radiation Induced Brachypodium Mutations in Cellulose Synthase By Tilling

Brachypodium distachyon has been focused as a new model plant for grass species. Brachypodium is a typical grass at the genome level, which exhibits an overall similarity of gene content and gene families when compared with rice and sorghum genomes. Therefore, Brachypodium is an excellent material for functional genomic studies in grass species. Targeting-Induced Local Lesions IN Genomes (TILLING) is an approach for reverse genetics study and has been wildly utilized to find induced mutation. For chronic irradiation, plants were subjected to irradiation at doses of 50, 100, 150, 200, and 250 Gy (total dose). Chronic irradiation was performed for 10 h every day, and lasted for 35 days. Total 384 M₂ mutants were used for TILLING library construction. Single nucleotide polymorphysms (SNPs) and/or insert and deletions (InDels) within genes related to lignocellulose biosynthesis were searched through TILLING analysis. SNPs were identified within the exon region of a genes responsible for cellulose synthesis. The analysis data could be used for functional genomics in the research of bioenergy crops. Acknowledgement: This research was supported by Radiation Technology R&D Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP)(NRF-2012M2A2A6035566).