P517
Physical Mapping of a QTL Region Affecting Resistance to Parasitic Infection in Yellowtail (Seriola quinqueradiata) By Chromosome Walking Using a BAC Library

Date: Monday, January 13, 2014
Room: Grand Exhibit Hall
Ryo Kumagai , Tokyo University of Marine Science and Technology, Tokyo, Japan
Junpei Suzuki , Tokyo University of Marine Science and Technology, Tokyo, Japan
Takashi Koyama , Tokyo University of Marine Science and Technology, Tokyo, Japan
Kazunori Yoshida , Seikai National Fisheries Research Institute, Fisheries Research Agency, Nagasaki, Japan
Akiyuki Ozaki , National Research Institute of Aquaculture - National Research Development Agency, Mie, Japan
Jun-ya Aoki , National Research Institute of Aquaculture - National Research Development Agency, Mie, Japan
Yumi Koda-Kawabata , NRIA, Fisheries Research Agency, Mie, Japan
Wataru Kai , NRIFS, Fisheries Research Agency, Kanagawa, Japan
Kanako Kobayashi-Fuji , Tokyo University of Marine Science and Technology, Tokyo, Japan
Satoshi Kubota , Tokyo University of Marine Science and Technology, Tokyo, Japan
Kazuki Akita , Tokyo University of Marine Science and Technology, Tokyo, Japan
Kazuo Araki , National Research Institute of Aquaculture - National Research Development Agency, Mie, Japan
Takashi Sakamoto , Tokyo University of Marine Science and Technology, Tokyo, Japan
The production of cultured yellowtail accounts for approximately 60% of marine finfish aquaculture in Japan. Benedenia disease caused by the ectoparasite Benedenia seriolaeis a serious parasitic disease in marine aquaculture finfish, leading to secondary viral or bacterial infections. Therefore, our group has been trying to establish benedenia disease resistant - strains by marker assisted selection (MAS) and to identify genes associated with benedenia disease resistance (BDR). We previously identified a major QTL associated with BDR on linkage group Squ2 (LOD score; 4.57, phenotypic variation explained (PVE); 20%, 95% confidence interval (95% CI); 5.5 cM). In this study, we attempted to make a physical map of this QTL region to search for candidate genes for BDR.

We constructed BAC contigs of the QTL region by chromosome walking using a yellowtail genomic BAC library. This QTL region was covered with 7 BAC clones. We then developed 18 microsatellite markers using a microsatellite-enriched library of the 7 BAC clones. QTL analysis with the new markers allowed us to narrow down the 95% CI for the QTL on Squ2 from 5.5 cM to 4.5 cM. We collected genomic sequences of the BAC contigs by next generation sequencing. BLAST searches of these sequences revealed some candidate genes that are involved in immune responses and that are expressed in fish skin.

Back to: Genome Mapping, Tagging & Characterization: Aquaculture
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