P516
Study of Sex-Determining System in Yellowtail, Seriola quinqueradiata

Date: Monday, January 13, 2014
Room: Grand Exhibit Hall
Takashi Koyama , Tokyo University of Marine Science and Technology, Tokyo, Japan
Akiyuki Ozaki , National Research Institute of Aquaculture - National Research Development Agency, Mie, Japan
Kazunori Yoshida , Seikai National Fisheries Research Institute, Fisheries Research Agency, Nagasaki, Japan
Junpei Suzuki , Tokyo University of Marine Science and Technology, Tokyo, Japan
Kanako Kobayashi-Fuji , Tokyo University of Marine Science and Technology, Tokyo, Japan
Jun-ya Aoki , National Research Institute of Aquaculture - National Research Development Agency, Mie, Japan
Wataru Kai , NRIFS, Fisheries Research Agency, Kanagawa, Japan
Yumi Koda-Kawabata , NRIA, Fisheries Research Agency, Mie, Japan
Tatsuo Tsuzaki , SNFRI, Fisheries Research Agency, Nagasaki, Japan
Kazuo Araki , National Research Institute of Aquaculture - National Research Development Agency, Mie, Japan
Takashi Sakamoto , Tokyo University of Marine Science and Technology, Tokyo, Japan
Since males and females are apparently indistinguishable but genetically distinct each other in yellowtail, a sex-linked genetic marker or ideally sex-determining gene is required for effective breeding program. Our previous effort identified the yellowtail sex-linked locus in linkage group 12 and their ZZ-ZW sex-determining system. In this study, to find potent sex-linked genetic markers and sex-determining gene, we further performed linkage analysis, developed BAC contig map, obtained whole sequence of the BACs and isolated single nucleotide polymorphism (SNP) markers. The minimum tiling path of the BAC contig consists of 10 clones and from the BAC-ends, two potent SNP markers for sex identification from wild population were developed. The sequence of the BAC contig represented 0.8 Mbp genomic segment. Association mapping and breakpoint analysis using several SNPs further developed from the BAC sequence showed approximately 80 kbp genomic segments as candidate region. BLAST search using in-house yellowtail ESTs and Nile tilapia transcriptome showed at least two potential sex-determining genes in this segment. Therefore, the SNP markers we developed in this study would be quite useful for sex-identification in yellowtail breeding program and our further efforts might identify sex-determining gene of yellowtail.
Back to: Genome Mapping, Tagging & Characterization: Aquaculture
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