P451Targeting a Resistance Gene Associated with Fusarium Wilt in Banana
Date: Monday, January 13, 2014
Room: Grand Exhibit Hall
Elizabeth Aitken
,
The University of Queensland, Brisbane, Australia
Sam Fraser Smith
,
University of Queensland, Brisbane, Australia
Manuel Matias Zander
,
University of Queensland, Brisbane, Australia
Jessica Dalton-Morgan
,
University of Queensland, Brisbane, Australia
Pradeep Ruperao
,
University of Western Australia, Brisbane, Australia
David Edwards
,
University of Queensland, Brisbane, Australia
Sharon Hamill
,
Queensland Dept of Agriculture, Fisheries and Forestry, Nambour, Australia
Mike K Smith
,
Queensland Dept of Agriculture, Fisheries and Forestry, Nambour, Australia
Jacqueline Batley
,
University of Queensland, Brisbane, Australia
The wild banana
Musa acuminata subsp
malaccensis (Malaccensis) has been reported to possess resistance to Fusarium wilt . We have assessed a population of Malaccensis plants which shows segregation for resistance to
Fusarium oxysporum f.sp.
cubense (
Foc)
. This segregation for resistance is not only to race 1, but also to sub-tropical race 4 (SR4) and the aggressive tropical race (TR4) of
Foc. All tests were undertaken in Australia in pot trials either in the glasshouse in Brisbane, Queensland, for race 1 and SR4, or under shade cloth in Darwin, Northern Territory in the case of TR4. After 10 weeks susceptible plants showed, corm splitting and leaf yellowing with internal symptoms of necrosis whereas resistant plants showed no symptoms at all.
Next generation sequencing is now being used to target regions associated with resistance. Next generation sequencing libraries have been prepared using bulks of the DNA from resistant and susceptible plants. Using the Illumina HiSeq, 100 bp paired end sequencing has been conducted to allow for 10x coverage for each bulk. SNP discovery using SGSAutoSNP has allowed for the identification of polymorphisms between the resistant and susceptible bulks. GWAS (Genome wide association study) analysis has revealed regions associated with resistance. The published Musa genome, has allowed the prediction of 96 NBS LRR genes across all chromosomes where we have identified high frequencies on Chromosomes 3, 6, 9 and 10. A significant region of association has been identified and any correlation with putative Fusarium resistance genes and these NBS-LRR is being identified. Concurrently, we are screening a larger segregating population to allow the option of fine mapping.
