P1146
Sequencing the MTP BAC Clones of Aegilops tauschii Chromosome Arm 3DS

Date: Monday, January 13, 2014
Room: Grand Exhibit Hall
Naxin Huo , Department of Plant Sciences, University of California, Davis, CA
Shuhong Ouyang , China Agricultural University, Beijing, China
Yi Wang , University of California, Davis, Davis, CA
Karin Deal , University of California, Davis, Davis, CA
Yong Liang , China Agricultural University, Beijing, China
Jianwei Zhang , University of Arizona
Mingcheng Luo , University of California, Davis, Davis, CA
Zhiyong Liu , China Agricultural University, Beijing, China
Qixin Sun , China Agricultural University, Beijing, China
Pat McGuire , Department of Plant Sciences, University of California, Davis, Davis, CA
Olin Anderson , USDA-ARS-WRRC, Albany, CA
Yong Q. Gu , USDA ARS, Western Regional Research Center, Albany, CA
Jan Dvorak , Department of Plant Sciences, University of California, Davis, CA
Wheat (Triticum aestivum) is one of the most important cereal crops worldwide. Polyploidy, huge size, and great complexity of the wheat genome make its sequencing challenging.  To assist with this endeavor and provide reference for future biological applications of the wheat genome sequence, we initiated sequencing the genome of Aegilops tauschii, the diploid progenitor of the wheat D genome, by employing the ordered-clone sequencing strategy. Compared to the whole genome shotgun (WGS) sequencing approach, this strategy, although more costly and labor intensive, will provide more accurate and reliable assembly. To exploit the high-throughputs of next generation sequencing (NGS) for sequencing of BAC clones, it is necessary to pool and index them with multiplex identifiers (MID).  Here we report sequencing of  3112 BAC clones of the MTP across the physical map of the Ae. tauschii chromosome arm 3DS .
Back to: Genome Sequencing & ESTs
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