Identification of QTLs Controlling Genes to Mn and Zn Uptake in Lentil Seed

Lentils are grown throughout the world. The world production of lentils is primarily from Canada, India, Turkey and Australia. Lentil seeds contain an excellient source of micronutrients which are essensial elements such as manganese (Mn) and zinc (Zn). These elements play important roles in the body for example; Mn for enzyme activation, hormones production and maintaining reproductive health and Zn for healthy skin, immune and reproductive function. The objective of these study was to determine inheritance of seed Mn and Zn accumulation in a recombinant inbred line (RIL) population of lentils from a cross (CDC-Redberry x ILL7502), which contain low x high Mn and Zn elements genotypes respectively, using a quantitative trait locus (QTL) mapping approach. Hundred and twenty lines were grown in three different location in Turkey. Mn and Zn concentrations in the seed were determined with using Atomic Absorption Spectroscopy. The range in Mn and Zn contents was found to be showing variation between 8,4 mg kg^-1 to 25,67 mg kg^-1 and 26,50 mg kg^-1 to 51 mg kg^-1 respectively. A linkage map comprising of 260 AFLP, SSR and SNP markers covering 843,5 cM was constructed in the RIL population. A total of 121 QTLs was identified throughout the map for Mn and Zn concentration in seed.