QTL Mapping of Genes Controlling to Fe Uptake in Lentil Seed (Lens culinaris L.) Using Recombinant Inbred Lines

Lentil is grown in area of 2,149,311 da and is produced about 302,181 tons in Turkey. The average yield of lentil is around 140 kg/da. Lentil is a good source of mitronutrient, especially iron. Lentil seed contains 40-90 mg/kg  Fe. Iron is an important diertary mineral that is involved in various body functions, including the transport of oxygen in the blood. All around the world, one of the most common micronutrient deficiency is iron deficiency, which causes anemia. Ability to store iron in seed is very important property and it should be understood the inheritance of seed iron accumulation.  Therefore, it is very important to identify and determine the genome localization of genes associated with uptake of iron in lentil seed. In this study, it was mapped genes controlling to Fe uptake in lentil. RIL population was developed from a cross between ILL 8006–BM (Barimasur-4) x CDC Milestone was used for mapping. These RILs were planted in three different locations (Izmir, Adana and S Urfa) of Turkey and, the seed Fe concentration was characterized using Atomic Absorption. The Fe values varied between 37 and 149 mg/kg among individuals of RIL. QTL mapping studies were performed in the RIL population using total of 181 markers, including 150 AFLPs, 27 SSRs and 4 SNPs. 149 markers were grouped into sixteen linkage groups covering 496,5 cM, with an average distance of 3,32 cM between markers at a LOD score of 3.0. Total of 4 QTLs were mapped in linkage groups of 1, 3, 6, and 7. Identification of these QTLs is very useful data for biofortification studies.