W209
Len Gen: The International Lentil Genome Sequencing Project

Date: Saturday, January 10, 2015
Time: 2:10 PM
Room: Pacific Salon 1
Kirstin Bett , University of Saskatchewan, Saskatoon, SK, Canada
Larissa Ramsay , University of Saskatchewan, Saskatoon, SK, Canada
Crystal Chan , University of Saskatchewan, Saskatoon, SK, Canada
Andrew G. Sharpe , National Research Council Canada / Global Institute for Food Security (U of S), Saskatoon, SK, Canada
Douglas R Cook , University of California-Davis, Davis, CA
R Varma Penmetsa , University of California at Davis, Davis, CA
Peter Chang , University of Southern California, Los Angeles, CA
Clare Coyne , USDA ARS, Pullman, WA
Rebecca McGee , Washington State University, Pullman, WA
Dorrie Main , Washington State University, Pullman, WA
Albert Vandenberg , University of Saskatchewan, Saskatoon, SK, Canada
We have been sequencing CDC Redberry using NGS of paired-end and mate-pair libraries over a wide range of sizes and technologies.  The most recent draft (v0.7) of approximately 150x coverage produced scaffolds covering over half the genome (2.7 Gb of the expected 4.3 Gb). Long reads from PacBio sequencing are being used to fill gaps and perform superscaffolding.  Genotype by sequencing (GBS) in key mapping populations is being used to anchor scaffolds as well as select individuals for re-sequencing.  Collaborations with labs in Australia, Czech Republic and ICARDA will facilitate further assembly and annotation of the genome as well as add to the growing database of genotypic diversity in the global lentil germplasm.  The sequence data from initial assemblies has been sufficient for the identification of gene sequences for traits of interest in the lentil breeding program and derived SNP markers are now being used in routine MAS.
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