W713
A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome

Date: Sunday, January 11, 2015
Time: 5:00 PM
Room: Golden Ballroom
Jarrod Chapman , DOE Joint Genome Institute, Walnut Creek, CA
Martin Mascher , IPK Gatersleben, Seeland, Germany
Aydın Buluç , Department of Energy Joint Genome Institute, Walnut Creek, CA
Kerrie W. Barry , DOE Joint Genome Institute, Walnut Creek, CA
Evangelos Georganas , Lawrence Berkeley National Laboratory, Berkeley, CA
Adam Session , University of California, Berkeley, CA
Veronika Strnadova , Department of Computer Science, University of California, Santa Barbara, CA
Jerry Jenkins , DOE Joint Genome Institute, Huntsville, AL
Sunish K. Sehgal , Kansas State University, Manhattan, KS
Leonid Oliker , Lawrence Berkeley National Laboratory, Berkeley, CA
Jeremy Schmutz , HudsonAlpha Institute for Biotechnology, Huntsville, AL
Katherine Yelick , Lawrence Berkeley National Laboratory, Berkeley, CA
Uwe Scholz , Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
Robbie Waugh , The James Hutton Institute, Dundee, United Kingdom
Jesse Poland , Kansas State University, Manhattan, KS
Gary J. Muehlbauer , Department of Plant Biology, University of Minnesota, St. Paul, MN
Nils Stein , Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Stadt Seeland, Germany
Daniel S. Rokhsar , DOE Joint Genome Institute, Walnut Creek, CA
Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive de novo a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat (Triticum aestivum) and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a shotgun assembly of sorted chromosome arms. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.
Back to: Sequencing Complex Genomes
<< Previous Abstract | Next Abstract >>