W474
BioNano Genome Map to Facilitate the Assembly of the Aegilops tauschii Genome

Date: Tuesday, January 13, 2015
Time: 3:50 PM
Room: Pacific Salon 3
Ming-Cheng Luo , Department of Plant Sciences, University of California, Davis, CA
Tingting Zhu , Department of Plant Sciences, University of California, Davis, Davis, CA
Karin R. Deal , Department of Plant Sciences, University of California, Davis, Davis, CA
Armond Murray , Department of Plant Sciences, University of California, Davis, CA
Sonny Van , Department of Plant Sciences, University of California, Davis, CA
Thanh C. Ngo , Department of Plant Sciences, University of California, Davis, CA
Shuyang Liu , Department of Plant Sciences, University of California, Davis, CA
Juan C. Rodriguez , Department of Plant Sciences, University of California, Davis, CA
Hai Long , Department of Plant Sciences, University of California, Davis, CA
Lichan Xiao , Department of Plant Sciences, University of California, Davis, CA
Naxin Huo , Department of Plant Sciences, University of California, Davis, CA
Luis Curiel , Department of Plant Sciences, University of California, Davis, CA
Luxia Yuan , Department of Plant Sciences, University of California, Davis, CA
Yi Wang , Department of Plant Sciences, University of California, Davis, CA
Yong Q. Gu , USDA ARS, Western Regional Research Center, Albany, CA
Olin Anderson , USDA ARS, Western Regional Research Center, Albany, CA
Daniela Puiu , Johns Hopkins University, School of Medicine, Baltimore, MD
Geo Pertea , Johns Hopkins University, School of Medicine, Baltimore, MD
Steven L Salzberg , Johns Hopkins University, School of Medicine, Baltimore, MD
Shuhong Ouyang , China Agricultural University, Beijing, China
Zhenzhong Wang , China Agricultural University, Beijing, China
Yong Liang , China Agricultural University, Beijing, China
Qixin Sun , China Agricultural University, Beijing, China
Zhiyong Liu , China Agricultural University, Beijing, China
Long Mao , Chinese Academy of Agricultural Sciences, Beijing, China
Zhengqiang Ma , Nanjing Agricultural University, Nanjing, China
Jan Dvorak , Department of Plant Sciences, University of California, Davis, CA
Aegilops tauschii is the donor of the D genome of hexaploid wheat (Triticum aestivum). Its large genome size and complexity impose a big challenge on sequencing and assembling the genome. Taking advantage of the available BAC-based physical map for the genome, a clone-by-clone sequencing approach is being used to generate a reference diploid genome sequence that will also be relevant for the wheat genomes. We will present the details of our strategy and technique for using a BioNano genome map to validate and rectify BAC sequence assemblies, and to build pseudomolecules for the chromosomes of the genome. This work is part of the NSF-funded Project IOS-1238231 to generate a reference sequence for the genome of Ae. tauschii (http://aegilops.wheat.ucdavis.edu/ATGSP/).
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