P0865
A Map-Based Cloning Approach for the Identification of a Low Temperature Sensitive Gene Sy-2 in Chilli Pepper (Capsicum chinense)

Date: Monday, January 12, 2015
Room:
LI LIU , Department of Plant Science and Plant Genomics Breeding Institute, Seoul National University and Vegetable Breeding Research Center, Seoul, South Korea
Jin-Ho Kang , Department of Plant Science and Plant Genomics Breeding Institute, Seoul National University and Vegetable Breeding Research Center, Seoul, South Korea
Yeong Deuk Jo , Department of Plant Science and Plant Genomics Breeding Institute, Seoul National University and Vegetable Breeding Research Center, Seoul, South Korea
Sota Koeda , Kyoto University, Kyoto, Japan
Munetaka Hosokawa , Kyoto University, Kyoto, Japan
Doil Choi , Department of Plant Science, Plant Genomics and Breeding Institute and Interdisciplinary Program in Agricultural Genomics, Seoul National University, Seoul, South Korea, Seoul, South Korea
Byoung-Cheorl Kang , Department of Plant Science and Plant Genomics Breeding Institute, Seoul National University and Vegetable Breeding Research Center, Seoul, South Korea
sy-2 (Seychelles-2) is a temperature sensitive natural mutant of Capsicum chinense and native to Seychelles Island in Africa. Previously we showed that sy-2 leaves were irregularly shaped and defective in chlorophyll development at temperatures below 24°C. A segregation test revealed that the sy-2 gene is controlled by a single recessive gene. To identify the sy-2 gene, we performed a map-based cloning approach using a total 600 individual F2 plants derived from crossing sy-2 and the wild type C. chinense ‘No.3341’. Fine-mapping of the locus allowed us to position sy-2 to an approximately 170-kb region flanked by markers IN2-1-1 and SNP-3-7 on chromosome 1. Among the approximately 36 hypothetical genes in this region several candidate genes including: HSP90-like ATPase family proteins, lipid-transfer proteins, calmodulin-domain protein kinases, and zinc finger proteins (ZFPs) were identified. RT-PCR and sequencing of the hypothetical genes are under way to identify sy-2.
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