Optical Mapping of the Solanum arcanum Genome

Genome reconstruction of Solanum arcanum

Although advances in next-generation sequencing technology for the use of extant germplasm resources now allow assembly of large numbers of complex genomes relatively rapidly and cheaply, they do not yet allow full genome reconstruction of the Solanaceae family, and hence are of limited use in breeding. The identification of compatible genomes for introgression breeding, the rearrangement phylogeny within the Solanaceae, and reconstruction of the acestral karyotype all require additional physical mapping information in addition to the genome sequence information to properly order contigs along the chromosome arms. Therefore, we have initiated the integration of next-generation sequencing and optical mapping data to advance on Solanum genome reconstruction. Here we report on de novo optical map and hybrid assemblies for Solanum arcanum.