Using the Wheat Tilling Database to Search Mutants of Interest

The exome sequencing of population comprised of 1000 EMS mutagenized tetraploid wheat lines for in silico targeting of induced local legions in genomes (TILLING) provides an invaluable reverse genetics tool for wheat researchers.  With a high probability to knockout a given gene, there are many lines with knockouts in targeted genes of interest.  This computer demonstration will lead researchers on how to use this new tool to rapidly identify wheat genes of interest, mutations that are available, and seed stock information.  During the session, we will cover BLASTing a gene of interest, finding potential high quality hits, and visualizing the results on a reference genome browser (JBrowse).  For each annotated gene, we have calculated mutation effects including synonymous and intergenic changes, to more harsh non-synonoymous, stop-gained, stop-loss, and splice site mutations.  This resource will be publicly available from the UCDavis- Dubcovsky Lab at:  http://dubcovskylab.ucdavis.edu/wheat-tilling