IDeal Fast ID Kit for DNA Extractions from Saliva Samples

The Ideal DNA Direct Lyse kit is an Ideal kit for molecular biologist that yields high quantities of DNA, with high DNA quality at a low cost in a relatively low time with few steps  for various downstream molecular applications including quantitative Real Time PCR (qPCR).

Saliva testing has great potential as a diagnostic fluid especially in periodontal diseases. We isolated DNA from Saliva samples using the IDeal kit and checked the DNA quality in qPCR with universal primers that  amplify the 16S ribosomal RNA gene. We found both the gram positive and gram negative species tested yielded good  amplification, comparable with magnetic silica extraction method.

The IDeal kit does not require organic extraction or ethanol precipitation and uses a lysis step and centrifugation step in the same tube avoiding transfers. The obtained supernatant was ready for qPCR. This simplistic procedure makes it economical and rapid for high throughput DNA extractions especially in 96-well plate format. The low cost per reaction of this kit makes it very attractive kit in the market.