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Genome Technology: High-throughput Methods

Full-Length cDNA Sequencing for Genome Annotation and Analysis of Alternative Splicing
Tyson A. Clark, Pacific Biosciences; Bo Wang, Cold Spring Harbor Laboratory; Ting Hon, Pacific Biosciences; Elizabeth Tseng, Pacific Biosciences; Michael Regulski, Cold Spring Harbor Laboratory; Doreen Ware, Cold Spring Harbor Laboratory/USDA-ARS

Validation of MO BIO’s PowerMag Soil DNA Isolation Kit on the Automated Liquid Handling VERSA 1100 Workstation
Sikander Gill, Aurora Biomed Inc; Rajwant Gill, Aurora Biomed Inc; Matthew Lemay, University of British Columbia; Dong Liang, Aurora Biomed Inc

Assembling Whole Genomes from Mixed Microbial Communities Using Hi-C
Ivan Liachko, University of Washington - Department of Genome Sciences

IDeal Fast ID Kit for DNA Extractions from Saliva Samples
Pranav Chhaliyil, Genetic-ID; Bernd Schoel, Genetic ID NA, Inc; Pradheep Chhalliyil, Genetic-ID

Tandem Repeat Detection Using Next-Generation Mapping and Its Application in Detecting rDNA Copy Number Variation in Plant and Human Genomes
Xiang Zhou, BioNano Genomics; Saki Chan, BioNano Genomics; Jinghua Shi, BioNano Genomics; Weiping Wang, BioNano Genomics; Zhanyang Zhu, BioNano Genomics; Alex Hastie, BioNano Genomics; Zeljko J. Dzakula, BioNano Genomics; Han Cao, BioNano Genomics

From PhénoBlé to AirPhen : Field Phenotyping Technologies Take Off
David Gouache, ARVALIS; Benoit de Solan, ARVALIS - Institut du végétal; Fred Baret, INRA; Alexis Comar, HiPhen

Data Management and Visualization in Digital Phenotyping
Christiaan Biemond, KeyGene; Gert-Jan Speckmann, KeyGene; Maarten Hekkelman, KeyGene NV; Koen Huvenaars, KeyGene NV; Timo Doeswijk, Keygene NV; Marco van Schriek, KeyGene NV; Sireen Khan, KeyGene

Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the Agilent 4200 TapeStation System
Rainer Nitsche, Agilent Technologies; Solange Borg, Agilent Technologies

Paramagnetic Cellulose DNA Isolation Improves DNA Yield and Quality Among Diverse Plant Taxa
Jackson Moeller, Department of Botany. UW-Madison; Nick R Moehn, Department of Botany. UW-Madison; Thomas J Givnish, Department of Botany. UW-Madison; Don M Waller, Department of Botany. UW-Madison

Computer Vision for Digital Phenotype Measures In Livestock
M.J. Woodward-Greene, USDA-ARS; Jason M. Kinser, George Mason University; Tad Sonstegard, Acceligen Inc. Animal Ag. Subsidary of Recombinetics; Heather J Huson, Cornell University; Johann Soelkner, University of Natural Resources and Life Sciences; Iosif Vaisman, George Mason University; Curtis P. VanTassell, Animal Genomics and Improvement Laboratory, USDA-ARS

NGS Library Preparation Method for Transcriptome Profiling With Enhanced Sensitivity of Transcript Detection
Vaishnavi Panchapakesa, New England Biolabs; Daniela B Munafo, New England Biolabs; Deyra N. Rodriguez, New England Biolabs; Erbay Yigit, New England Biolabs; Mehmet Karaca, New England Biolabs; Pingfang Liu, New England Biolabs; Lynne M. Apone, New England Biolabs; Christine J. Sumner, New England Biolabs; Christine Chater, New England Biolabs; Laurie Mazzola, New England Biolabs; Joanna Bybee, New England Biolabs; Danielle Rivizzigno, New England Biolabs; Fiona J Stewart, New England Biolabs; Eileen T Dimalanta, New England Biolabs; Theodore B Davis, New England Biolabs

Development of Automated DNA and RNA Plant Extraction Kits Using the New Maxwell® RSC Platform
Chris Moreland, Promega Corporation; Lyndsey Jager, Promega Corporation; Samantha Lewis, Promega Corporation; Brad Hook, Promega Corporation; Adam Blatter, Promega Corporation; Cristopher Cowan, Promega Corporation

Advances in Small RNA Library Preparation Allow Combination of Bias Reduction with Gel-free or Low Input Protocols
Dawn Obermoeller, Bioo Scientific; Masoud Toloue, Bioo Scientific; Adam Morris, Bioo Scientific; Kevin Allen, Bioo Scientific; Suk Ho Eun, Bioo Scientific

High PCR Efficiency Using Ideal DNA Extraction Kit
Pradheep Chhalliyil, Genetic-ID; Bernd Schoel, Genetic ID NA, Inc

Evolinc: A Pipeline for Comparative Genomic and Transcriptomic Analyses of Long Non-Coding RNAs
Andrew D Nelson, School of Plant Sciences, University of Arizona; Upendra K Devisetty, Oregon State University; Asher K Haug-Baltzell, University of Arizona; Evan S Forsythe, School of Plant Sciences, University of Arizona; Eric Lyons, University of Arizona; Mark A Beilstein, School of Plant Sciences, University of Arizona

Performance of a Multi-Species-Plant Illumina BeadChip
Marie-Christine Le Paslier, INRA, US1279 EPGV-Etude du Polymorphisme des Génomes Végétaux, CEA-IG/Centre National de Génotypage; Aurélie Bérard, INRA, US1279 EPGV-Etude du Polymorphisme des Génomes Végétaux, CEA-IG/Centre National de Génotypage; Aurélie Chauveau, INRA, US1279 EPGV-Etude du Polymorphisme des Génomes Végétaux, CEA-IG/Centre National de Génotypage; Elodie Marquand, INRA, US1279 EPGV-Etude du Polymorphisme des Génomes Végétaux, CEA-IG/Centre National de Génotypage; Anne Boland-Auge, CEA-Intitut de Génomique/Centre National de Génotypage; André Eggen, Illumina, AgriGenomics; Dominique Brunel, INRA, US1279 Etude du Polymorphisme des Génomes Végétaux, CEA-IG / Centre National de Génotypage; Patricia Faivre Rampant, INRA, US1279 EPGV-Etude du Polymorphisme des Génomes Végétaux, CEA-IG/Centre National de Génotypage

Plant Virus Identification and Detection Using High-Throughput Sequencing
Michel Ebskamp, Naktuinbouw; Ellis Meekes, Naktuinbouw; Iris Stulemeijer, BKD; Maarten de Kock, BKD; Fleur Gawehns-Bruning, Naktuinbouw

Digital PCR Efficiency of Stool and Soil Samples Using Ideal DNA Extraction Kit
Bernd Schoel, Genetic ID NA, Inc; Pradheep Chhalliyil, Genetic-ID

Quantitative Genotyping from GBS Data in Autopolyploids
Guilherme Da Silva Pereira, North Carolina State University; Gabriel Rodrigues Alves Margarido, University of São Paulo; Marcelo Molinari, University of São Paulo; Antonio Augusto Franco Garcia, University of São Paulo; Zhao-Bang Zeng, North Carolina State University

A High-Throughput and High-Resolution Microfluidics System for SNP Genotyping
Jing Wang, Fluidigm Corp; Jian Qin, Fluidigm Corp; Janet Ziegle, Fluidigm Corp; Ramesh Ramakrishnan, Fluidigm Corp

High Quality Library Construction and Reliable Quantitation with NEBNext Reagents
Pingfang Liu, New England Biolabs; Lynne M. Apone, New England Biolabs; Nathan A. Tanner, New England Biolabs; Janine G. Borgaro, New England Biolabs; Vaishnavi Panchapakesa, New England Biolabs; Deyra Rodriguez, New England Biolabs; Erbay Yigit, New England Biolabs; Bradley W Langhorst, New England Biolabs; Don Johnson, New England Biolabs; Julie F. Menin, New England Biolabs; Christine J. Sumner, New England Biolabs; Christine Chater, New England Biolabs; Fiona J Stewart, New England Biolabs; Laurie Mazzola, New England Biolabs; Joanna Bybee, New England Biolabs; Danielle Rivizzigno, New England Biolabs; Nicole M. Nichols, New England Biolabs; Eileen T Dimalanta, New England Biolabs; Theodore B Davis, New England Biolabs

Direct Target Enrichment, a Hybridization-Based Capture Method for Selective Acquisition of NGS Data
Sarah K. Bowman, Directed Genomics; Amy Emerman, Directed Genomics; Daniel Kraushaar, Directed Genomics; Kruti M. Patel, Directed Genomics; Noa Henig, Directed Genomics; Salvatore Russello, New England Biolabs; Theodore B Davis, New England Biolabs; Cynthia L. Hendrickson, Directed Genomics

Comprehensive, Cost Competitive, Genomics Capabilities at The McDonnell Genome Institute at Washington University
Robert Fulton, McDonnell Genome Institute at Washington University; Vincent Magrini, McDonnell Genome Institute at Washington University; Daniel Koboldt, McDonnell Genome Institute at Washington University; Wesley Warren, McDonnell Genome Institute at Washington University; Richard K Wilson, McDonnell Genome Institute at Washington University

Improved Signal-to-Noise Ratios in qPCR Assays for Genomic Copy Number Variation (CNV) Determination Using Dual-Quenched BHQ® Probes
James Flynn, LGC Biosearch Technologies; Albert Wong, LGC Biosearch Technologies; Luan Le, LGC Biosearch Technologies; Ben Sowers, LGC Biosearch Technologies

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