P0220
Digital PCR Efficiency of Stool and Soil Samples Using Ideal DNA Extraction Kit

Bernd Schoel , Genetic ID NA, Inc, Fairfield, IA
Pradheep Chhalliyil , Genetic-ID, Genetic-ID Inc, IA
Digital PCR is a promising technology in  microbial research and infectious disease management, especially in characterizing changes in microbiomes. However, the presence of PCR inhibitors, cost and quick DNA extraction limits the use of dPCR.

The IDeal DNA extraction kit  is an ideal kit for digital pcr. Here we present the extracted DNA in digital droplet PCR using inhibitors rich stool and soil samples.

The IDeal kit. does not require organic extraction or ethanol precipitation or using any time consuming binding columns or magnetic silica and is suitable for many downstream applications.  This makes, the IDeal kit.  isolation procedure a  simple, economical and rapid kit which is also available in 96-well plate format for high throughput analysis..

The low cost per reaction of this kit and minimum loss of DNA makes it very attractive kit in the market.