Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the Agilent 4200 TapeStation System

The Agilent 4200 TapeStation system provides automated, fast and reliable DNA and RNA electrophoresis for up to 96 samples using pre-packaged reagents and minimal manual handling. The Agilent D5000 and High Sensitivity D5000 ScreenTape assays have been developed for the separation and analysis of DNA fragments from 100 bp to 5000 bp, a size range that complements and resides between the D1000 ScreenTape and the Genomic DNA ScreenTape assays. The Agilent 4200 TapeStation system and the DNA ScreenTape assays can be used at several steps of the Next Generation Sequencing (NGS) workflow. With the emergence of new methodologies, such as the use of Transposomes, NGS library sizes are tending to increase beyond 1000 bp, even for the short-read NGS technologies.

This Poster focuses on the performance of both D5000 ScreenTape assays with respect to the accuracy and precision of quantification and sizing, as well as the sensitivity of these assays. Data analysis for quantification and molarity determination further directly compared to the corresponding assay for the Agilent 2100 Bioanalyzer system. Additionally, performance of both D5000 and High Sensitivity D5000 assays on the 4200 TapeStation was compared to the 2200 TapeStation system.