P0216
High PCR Efficiency Using Ideal DNA Extraction Kit

Pradheep Chhalliyil , Genetic-ID, Genetic-ID Inc, IA
Bernd Schoel , Genetic ID NA, Inc, Fairfield, IA
DNA loss in varying percentages is inevitable in most DNA extraction methods. The IDeal  DNA extraction kit is ideal to be used with very small sample sizes especially with valuable samples. In this kit the lysed extract can be used without much loss with high quality DNA for many molecular biology application.

 Here we present qPCR data using Ideal direct lyse DNA extraction kit, showing that DNA purified from animal tissue (mouse tail), bovine blood, formalin-fixed paraffin-embedded tissue, soil, cigarette  and herbarium samples.   The  superior quality using the IDeal kit is better than the  other commercial Direct Lyse DNA extraction kits.  The most consistent Relative PCR Efficiency (RPE), a measure of suitability of purified DNA for qPCR, was observed with DNA purified using the IDeal kit.

 The IDeal kit. does not require organic extraction or ethanol precipitation and is suitable for many downstream applications. In addition, the IDeal kit.  isolation procedure is simple, economical and rapid. For high throughput DNA extraction, the IDEAL kit is currently available in 96-well plate format.

The low cost per reaction of this kit and minimum loss of DNA makes it very attractive kit in the market.

Handouts
  • Fast ID-poster-2-PRN.pdf (6.9 MB)