High Quality Draft Genomes of Medicago truncatula & Gossypium herbaceum using NGS, Dovetail & BioNano Technologies

Here we present a high quality draft genome of Medicago truncatula (HM340), a model plant for studying legume symbioses and Gossypium herbaceum (cv. Wagad), a A-genome diploid from the Old World with genome sizes of approximately 0.5 & 1.7 Gbp respectively using a multi-platform sequencing strategy (Illumina HiSeq, PacBio RS II, Dovetail Genomics, and BioNano Whole Genome Maps). The multi-platform sequencing strategy will help immensely in achieving high quality de novo reconstructions of genomes, which requires accurate long-range contiguity. Using hybrid sequencing and analysis will not only provide high quality reference genomes which is a necessary foundation for several biological discoveries but also will aid in assessing recent technologies such as Dovetail Genomics and BioNano Genomics and also serve as a model to the plant genomics community who has an interest in using multi-platform sequencing technologies for de novo genome sequencing.