Designing a bait library for NLR genes in Triticeae

Many resistance genes in plants encode for proteins with a nucleotide binding site and leucine rich repeats. A plant genome harbours several hundred of these genes.

We use resistance gene enrichment sequencing (RenSeq) to target this gene family. RenSeq is typically applied in gene cloning projects that involve an EMS mutant screen or bulk segregant analysis. Additionally, the target genes often originate from wild relatives of crops.

Therefore on the one hand a bait library needs to be as inclusive as possible, but on the other hand it needs to have as little redundancy as possible.

In this presentation we will focus on the step-by-step design of a RenSeq bait library for Triticeae.