Sequencing and Functional Annotation of the Transcriptome of Oncorhynchus kisutch

Oncorhynchus kisutch (Coho salmon), is an important anadromous aquaculture resource species and a valuable organism to ecological studies. However, compared with another salmonids species the genomics resources of coho salmon are rather scarce. Here, we develop a comprehensive transcriptome database of coho salmon using Illumina technology (HighSeq 2000). Over 394 million of reads were assembled producing 216,100 contigs with an average length of 653.36 and N50 of 1,014. Over the 13% of total contigs assembled presented at least one type of SSR motif. Using different tools we annotated over the 60% of the contigs and assigned 19.21% of GO categories and 8.73% of KEGG metabolic pathways. These results represent a valuable resource in future genomic and genetic studies in coho salmon.