Analysis of Quantitative Trait Loci of Allelopathic Trait in Rice

In this study, allelopathy of F8 RILs in Nongan(low allelopathic cultivar) X Sathi(high allelopathic cultivar) were screened using ‘ECAM(Equal Compartment Agar Method)’. Incu Tissue (72x72x100mm) filled with 30mL of 0.3% nutrient-free water agar were sown with the germinated seedlings of each rice cultivar. After 1 week of growth, germinated lettuce seedlings were added to the other half of the Incu Tissue. The lettuce seedlings were removed after one week of co-existence with the rice seedlings and measured root length, shoot length, root weight, shoot weight. Using this data we made a distribution chart and it appeared normal distribution. In order to identify the gene related to allelopathy, total 56 SSR marker distributed through 12 chromosomes were conducted to interval mapping. One major QTL was on chromosome 3 and three minor QTL were on chromosome on chromosome 5, 9, 12. For fine-mapping of QTL, for the analysis of quantitative trait loci. These SSR markers are linked on 12 chromosomes in rice. QTL related to high allelopathic potential similarly with sathi were between RM143 and RM517 on chromosome 3. Q-seq of the 10 highest allelopathic lines and the 10 lowest allelopathic lines will be presented.