Detection of a Genetic Locus, Plays a Role in Determining High Amylose Content in Rice Using Whole Genome Re-Sequencing

Goami2, mutant cultivar mediated by NMU, is well known as its extinguishing endosperm characteristics. One of the most extraordinary endosperm characteristic is high level of amylose content, even though the hulled rice is totally opaque. There have been many studies to address the unique physico-chemical properties and possible usages as a healthy and functional food ingredient, especially, the high-amylose rice had a positive effect on lowering the blood glucose response in obesity and type 2 diabetes. Genetic linkage map was constructed based on the genotypes of 112 F₂ progenies derived from Goami2 (japonica) / Milyang23 (indica) by using a total 324 SSR and SNP markers. The high amylose content of Goami2 was directed by a major recessive locus located in the 19.34-19.70Mbp region of chromosome 2. Out of a hundreds SNP within Goami2 and its wild type (Ilpum) on candidate region, we developed a CAPs marker entirely co-segregated with high amylose content.