Using the Wheat Tilling Resource to Find Mutations of Interest

The exome sequencing of a population comprised of 1500 EMS mutagenized tetraploid wheat lines (cv. Kronos) and 1200 mutagenized hexaploid wheat lines (cv. Cadenza) for in silico targeting of induced local legions in genomes (TILLING) provides an invaluable reverse genetics tool for wheat researchers. With a high probability to knockout a given gene, there are many lines with knockouts in targeted genes of interest. During the session, we will cover finding a gene of interest through our BLAST tool, finding potential high quality hits, then visualizing the results on a reference genome browser (JBrowse), and submitting an order form to request the seeds. For each annotated gene, we have calculated mutation effects for synonymous and intergenic changes, but also non-synonoymous, stop-gained, stop-loss, and splice site mutations. This resource will be publicly available from the UCDavis - Dubcovsky Lab at: http://dubcovskylab.ucdavis.edu/wheat-tilling