The Latest in DNA Library Preparation: Superior Performance from Picogram Input Amounts with Nebnext® Ultra™ II, Plus New Enzymatic DNA Fragmentation.

With its unprecedented throughput, scalability, and speed, next-generation sequencing (NGS) is expanding its applications; addressing an increasingly diverse range of biological problems. As the use of Next Generation Sequencing (NGS) is becoming more widespread there is a need for faster protocols that perform reliably even with reduced input amounts. To address these challenges the new NEBNext^® Ultra™ II library prep solutions have optimized every step in the workflows to enable high quality of sequencing data even from few picograms (gDNA) and low nanograms (RNA) of input material. Enzymatic fragmentation has also been included to streamline the library prep workflow and to avoid the sample lost involved in mechanical shearing.